In-vitro α-amylase and α-glucosidase inhibitory activity of apple peel extract (APE)

Author: Sandesh P. Kamdi, Amit Raval and Kartik T. Nakhate

1. Faculty of Pharmacy, Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan, India. 2. Department of Pharmacology, Rungta College of Pharmaceutical Sciences and Research, Bhilai 490024, Chhattisgarh, India

Abstract

The present study was to investigate an in-vitro potential inhibitory activity of extracts and fractions of Apple peel extract (APE) on α-amylase and α-glucosidase enzymes. The APE were prepared from Lal Ambri (Red Delicious) Apple (Maluspumila), first with cold maceration (70% v/v ethanol) and then by Soxhlation techniques (95% v/v ethanol). Different concentrations (0.1, 0.2, 0.3, 0.4, and 0.5 mg/ml) of extract and fractions were subjected to α-amylase and α-glucosidase inhibitory assay. The absorbance was measured at 540 and 405 nm using multiplate reader and the percentage of α- amylase and α- glucosidase inhibitory activity and IC50 values of extract and fractions were calculated. Acarbose was used as a standard reference drug, which showed α-amylase inhibitory activity with an IC50 value of 0.15 mg/ml and α-glucosidase inhibitory activity with an IC50 value of 0.98 mg/ml. Amongst all, fraction 2 of APE has shown best enzyme inhibitory activity with an IC50 value 0.21 (α-amylase and α-glucosidase) and 0.18 mg/ml respectively which were comparable with that of acarbose. The results of the present study indicate that, fraction 2 of APE, rich in flavonoids and polyphenols, are effective α- amylase and α- glucosidase inhibitors, which may be helpful to reduce the postprandial glucose levels. Hence, further studies may throw light on the antidiabetic potential of APE especially in the management of type 2 diabetes.

Key words: Apple peel extract, α- amylase, α-glucosidase, type 2 diabetes


Mr. Sandesh P. Kamdi
Research Scholar, Faculty of Pharmacy, Pacific Academy of Higher Education and Research University, P.B-12 Pacific Hills, Airport Road, Debari, Udaipur313024, India. Email: sandeshkamdi@gmail.com
Article Type : Research Article
Date : 2019-11-16
No of Downloads : 45